Product Description
REAGEN™Zilpaterol Strip Test is a qualitative and rapid lateral flow assay designed to detect zilpaterol residues in urine, feed and milk sample.
The unique features of the kit are:
l Rapid strip test method – 12 minutes
lNovel binding protein that requires no heating step
l High sensitivity，3-5ppb
lHigh reproducibility
Mechanism of Reaction
REAGEN™ The assay uses a competitive colloidal gold based format. The extration sample (100 μL) is added to a clear plastic reaction vessel, and used to resuspend the lyophilized reagents to a uniform pink color in the bottom of the microtiter wells. The sample is incubated briefly (4 min) to allowed to flow up the strip for 8 minutes by capillary action. The anti-zilpaterol antibodies on the gold particles engage with any Zilpaterol present in the sample. Any gold particles that are not complexed with zilpaterol present in the sample will bind to the zilpaterol imprinted at the Test line (T-line), forming a signal (red line) at that position. If the Anti-zilpaterol antibodies on the gold particles have engaged with zilpaterol present in the sample, the gold particle will flow past the T-line and reach the Control line (C-line). For visual interpretation of the test results, T-line signal intensity that is stronger or equal to the signal at the C-line indicates a negative result. Signal at the T-line which is less intense than the C-line indicates the presence of trace levels of zilpaterol in the sample (≥3-5 ppb*dilution factor)
Kit Contents, Storage and Shelf Life
The REAGEN™ Zilpaterol Strip Test has the capacity for 96(RNS92015).The shelf life is 12 months when the kit is properly stored.
Kit Contents | Amount | Storage |
Reaction Wells | 12T x8 (96 total) | RT(25˚C+/- 2.5) (Dry,dark and well sealed ) |
Test Strips | 48T x2 (96 total) | |
Microplate | 1 | RT(25˚C+/- 2.5) |
Extration Buffer | 1 vial(28mL) | |
Manual | 1 |
A 96-wells plastic frame is also provided in each kit to anchor the reaction wells in place during testing, and can be re-used as required.
Sensitivity (Detection Limit)
Milk | ≥3-5ppb | Test line obvious weaker than control line |
Bovine and swine urine | ≥3-5ppb | Test line obvious weaker than control line |
Bovine and swine feed | ≥18-30ppb | Test line obvious weaker than control line |
Required Materials Not Provided With the Kit
l Timer
l Additional positive and negative controls
l Polypropylene tubes
l Centrifuge (or filter paper)
l Distilled water
l Scissors to cut strip caps
l Disposable fixed volume pipettor (200 µL and 1000 µL)
l 2Mol/L NaOH
Warnings and Precautions
l Do not use the kit past the expiration date.
l Do not re-use pipette tips or polypropylene tubes.
l Be sure unused wells remain tightly sealed with the plug caps.
l Incubations of assay should be timed as precisely as possible
l Always store test strips in provided vials to maintain stability.
l Positive samples should always be re-tested alongside a positive and negative control.
