Real-time PCR

REAGEN LLC 85

Real-time PCR

Real-time PCR

Molecular detection methods have revolutionized medical diagnosis and are an essential part of todays laboratory diagnosis.

 

In many areas of infectious diseases, the classical detection methods such as culture and immunoassays have been replaced or supplemented by real-time polymerase chain reaction (PCR).



  • Real-time PCR test principle


By direct detection of DNA or RNA of the pathogen, real-time PCR allows for an early and highly specific diagnosis, thus leading to a fast and selective treatment regime of the individual patient; after DNA isolation, target gene fragments are amplified (amplicons) and, if present, detected with fluorescent hydrolysis probes. The fluorescence signal increases with the amount of formed amplicons which is detected by the optical unit of a real-time PCR instrument.



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REAGEN LLC,focusing on ELISA kits including clinical disease detecting kits,food&feed safety test kits and animal epidemic virus detecting kits in US for 6 years

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  • 721 W Whittier Blvd,     
             Unit S,La Habra,CA90631,USA
  • 619-389-8888
  • reagenkits@gmail.com
  • www.reagen.us